Culture of human endothelial and smooth muscle cells, Generation and culture of vascular organoids, Full range of molecular biology and protein biochemistry techniques, Expression profiling, RNAseq, Immunofluorescence-based analyses, Confocal microscopy, Investigate the impact of high glucose and oxLDL levels on the capacity of ECs to maintain the VSMC phenotype, Investigate the general influence of clinically relevant pharmacological inhibitors of prostanoid generation on the VSMC phenotype switch in a pro-arteriosclerotic environment, Investigate the role of prostacyclin-dependent signaling in this context
